Maybe should say it is a busy month too.
I do not want to waste my time anymore. So let's get to work.
An amount of bacterial hosts and expression vectors appearing in my minds now.
The classic - BL21(DE3) and BL21(DE3) pLysS.
The hybrid of origami and rossetta - Rosetta-gami2(DE3)
The NEB express - ER2523
+
pET32 Xa/Lic
pCold1 Xa
pMAL -c5x
pMAL -p5x
Good luck and all the best to me!
As well as the revival of glycerol stock of my precious Rosetta-gami2(DE3)pET32-PhaCcs which used to produce soluble PhaCcs.
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