No PhaC protein on the soluble fraction; but a estimated ~15% expressed PhaC in insoluble fraction. [Troubleshooting: I still got other stock, will sonicate other bacterial cells and check again]
Maybe it is better to subclone the stored plasmid to Rosetta-gami2 (DE3) and BL21 (DE3) , BL21 (DE3)/pLySs?
At the same time, design primer and clone into pCold and pMAL.
Will email Dr after I have troubleshoot for insolubility and plan the cloning experiments in this weekend.
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