Stuck @ dec 2010

E. coli Rosettagami2(DE3) with pET32 harbouring phaCcs gene

problem 1: Expression level of PhaC is very low compare to previous batches

(less than 10% , will be very difficult to be purified)

problem 2: Almost 100% of the expressed PhaC is insoluble

(not a native functional protein we want)

Troubleshoot:

1. IPTG is not functioning?

- trial: induce with IPTG from colleage which is functioning for their expression.