Friday, July 30, 2010

Optimization of gel filtration

These are the factors to be considered in order to optimize gel filtration resolution:
1. Sample Volume
2. Ratio of sample volume to column volume
3. Column dimensions
4. Particle size
5. Particle size distributions
6. Packing density
7. Pore size of the particles
8. Flow rate
9. Viscosity of the sample and buffer

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