JUNE-JULY

Was trying to produce more protein from the 1.6L induced bacterial cultures.

Purified with Affinity chromatography successfully. (Although lost an amount of protein due to my mistakes)

Concentrated it with a protein concentrator since it has been diluted.

Polishing with size exclusion chromatography.

The peak of elution is a bit broad although it is very high peak.

After SDS PAGE analysis, the results shown a thick fat band at around 80kDa, but problem is they are not alone. some contaminants found at size 30-60kDa.

Are they complex with PhaC? malfunction of the column? or any other possibilities?